WebCTCF. Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. [5] [6] CTCF is involved in many cellular processes, … WebAnti-CTCF Antibody serum, Upstate®; Synonyms: 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), …
Evidence for how a key transcription factor manages access to DNA
WebNov 3, 2014 · CTCF knockdown in mESCs was performed via lentiviral infection of shRNA as previously described (60, 61). ChIP and Western blot analysis were performed as … WebNov 6, 2014 · Sonicated chromatin was pre-cleared and incubated with 2 µg of anti-RAD21 antibody (ab992, Abcam), 4 µl anti-CTCF (07729, Millipore), 2 µg anti-PAX5 (sc-1974, Santa Cruz) and 1 µg anti-IgG (sc-2027, Santa Cruz) overnight. The complexes were washed with increasing salt stringency and cross-links were reversed in the presence of 0.2 M NaCl at ... on the market property chester le street
(07-729) Anti-CTCF Antibody - MilliporeSigma - CiteAb
WebSonicated chromatin was pre-cleared and incubated with 2 µg of anti-RAD21 antibody (ab992, Abcam), 4 µl anti-CTCF (07729, Millipore), 2 µg anti-PAX5 (sc-1974, Santa Cruz) and 1 µg anti-IgG (sc-2027, Santa Cruz) overnight. The complexes were washed with increasing salt stringency and cross-links were reversed in the presence of 0.2 M NaCl at ... WebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c … WebOct 6, 2024 · CTCF–CTCF loops and cohesion are both sensitive to RAD21 cleavage, pointing to similarities in the way cohesin associates with DNA as it stabilizes loops at CTCF sites and when it mediates ... on the market property fishguard