Phosphatase activity in mung beans
WebMung bean acid phosphatase reacted immunologically with primary antibodies against Solanum tuberosum acid phosphatase, while polymerase chain reaction (PCR) analyses suggest there are some common sequences between mung bean acid phosphatase DNA and that of Arabidopsis thaliana acid phosphatase vegetative storage protein. WebThe efficiency of different phosphatase-producing fungi in arid soils was studied in pot experiments in order to evaluate the effect of these organisms on the enhancement of rhizosphere enzyme activities, the uptake of different nutrients, dry matter production, and grain yields of the mung bean. A significant increase in phosphatase (acid and alkaline), …
Phosphatase activity in mung beans
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Webrhizosphere of mung bean and tested in lab for phosphate solubilization and phosphatase activities. Five bacteria were found positive for P solubilization as well as in vitro production of ...
WebThis paper describes the educational use and potential of an acid phosphatase enzyme extracted from mung beans (Phaseolus aureus), which the author has used in A-level … WebMung bean pyruvate kinase (PK) practically free from PEP-phosphatase has been purified about 36 fold. The enzyme is irreversibly inactivated on desalting by gel filtration or dialysis (without EDTA). The inactivation is also observed in the presence of ATP, Mg2+ or thiols but is prevented by a non-p …
Webthe thermostable nature of acid phosphatase isolated from germinated mung bean seeds. Effect of pH on acid phosphatase activity Acid phosphatase activity was recorded at … WebDec 1, 1991 · It is suggested that mung bean PEP-specific alkaline phosphatase activity is due to cytosolic pyruvate kinase, in which pyruvate and ortho-phosphate are formed in the …
WebMung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono and oligonucleotides carrying a 5′-P terminus. Mung bean nuclease …
WebFiltration of mung bean nuclease I on Sephadex G-100. The column (Phar- macia, 2.5 X 100 cm) was eqllilibratedwith 0.05 M Tris-HCl buffer, pH 8.9. The material obtained after … simple isn\\u0027t easy olivia goldsmithWebMung Bean Nuclease I III. PURIFICATION PROCEDURE AND (3’)~o-MONOPHOSPHATASE ACTIVITY* (Received for publication, May 8, 1970) A. J. MIKULSKI AND M. LASKOWSKI, SR. From The Laboratory of Enzymology, Roswell Park … simple isn\\u0027t easyWebOct 10, 1970 · Mung bean nuclease I of potency 1800 was obtained in small yield. At this stage the preparation showed two bands on disc electrophoresis. Throughout all stages of purification the nuclease... rawpressery.comWebDec 1, 1991 · Abstract. The procedure of Malhotra and Kayastha ([1990] Plant Physiology 93: 194-200) for the purification to homogeneity of a phosphoenolpyruvate-specific alkaline phosphatase (PEP phosphatase) from germinating mung beans (Vigna radiata) was followed.Although a higher specific activity of 1.4 micromoles pyruvate produced per … raw pressery cold coffeeWebNov 16, 2012 · In the present study, acid phosphatase enzyme was isolated and partially purified from germinated local mung bean seeds. The sequential partial purification … raw pressery acquisitionWebA phosphoenolpyruvate (PEP) phosphatase was purified to homogeneity from germinating mung beans (Vigna radiata). It was found to be a tetrameric protein (molecular mass 240,000 daltons) made up of apparently identical subunits (subunit molec-ular mass 60,000 daltons). It was free from bound nucleotides. It did not show pyruvate kinase activity. raw pressery detox reviewWebJan 4, 2012 · The activity of acid phosphatase secreted by the roots under phosphorus-deficient conditions was remarkably high in lupin and tomato, high in cabbage and radish, moderate in soybean and sugar beet, and low in wheat and azuki bean. The increased rates of activity of acid phosphatase (on a root dry weight basis) secreted under phosphorus ... simple islamic prayer